Proteus vulgaris: Difference between revisions
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==Description and significance== | ==Description and significance== | ||
P. vulgaris is rod-shaped gram-negative chemoheterotroph. It inhabits in gastrointestinal tracts of animal, soil, polluted water, raw meat, and dust. | ''P. vulgaris'' is rod-shaped gram-negative chemoheterotroph. It inhabits in gastrointestinal tracts of animal, soil, polluted water, raw meat, and dust.<br/> | ||
''Proteus'' can be isolated from a sample of soil. Organism is inoculated in a nutrient agar to form colonies. To test the gram-negative and oxidase-negative characteristics of ''Enterobacteriaceae'', gram stains and the oxidase test are performed. The colonies of interest are then inoculated onto a selective and differential medium-McConkey agar. McConkey agar is suitable for ''Proteus'' for two reasons. First reason is that the bile salt constituent allows the growth of ''Proteus'', which is a part of the intestinal flora, while selectively inhibits organisms that are not suitable to grow in interstinal environments. Second reason is that McConkey agar can differentiate the non lactose-fermenting characteristic of ''Proteus'' from the lactose-fermenting organisms. Since ''Proteus'' is an anaerobic organism, the plate agar can be incubated in an anaerobic jar. | |||
Describe the appearance, habitat, etc. of the organism, and why it is important enough to have its genome sequenced. Describe how and where it was isolated. | Describe the appearance, habitat, etc. of the organism, and why it is important enough to have its genome sequenced. Describe how and where it was isolated. | ||
Revision as of 19:46, 26 March 2008
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Classification
Higher order taxa
Domain: Bacteria
Phylum: Proteobacteria
Class: Gamma Proteobacteria
Order: Enterobacteriales
Family: Enterobacteriaceae
Species
P. vulgairs
Description and significance
P. vulgaris is rod-shaped gram-negative chemoheterotroph. It inhabits in gastrointestinal tracts of animal, soil, polluted water, raw meat, and dust.
Proteus can be isolated from a sample of soil. Organism is inoculated in a nutrient agar to form colonies. To test the gram-negative and oxidase-negative characteristics of Enterobacteriaceae, gram stains and the oxidase test are performed. The colonies of interest are then inoculated onto a selective and differential medium-McConkey agar. McConkey agar is suitable for Proteus for two reasons. First reason is that the bile salt constituent allows the growth of Proteus, which is a part of the intestinal flora, while selectively inhibits organisms that are not suitable to grow in interstinal environments. Second reason is that McConkey agar can differentiate the non lactose-fermenting characteristic of Proteus from the lactose-fermenting organisms. Since Proteus is an anaerobic organism, the plate agar can be incubated in an anaerobic jar.
Describe the appearance, habitat, etc. of the organism, and why it is important enough to have its genome sequenced. Describe how and where it was isolated. Include a picture or two (with sources) if you can find them.
Genome structure
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Does it have any plasmids? Are they important to the organism's lifestyle?
Cell structure and metabolism
Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.
Ecology
Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.
Pathology
How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
Application to Biotechnology
Does this organism produce any useful compounds or enzymes? What are they and how are they used?
Current Research
Enter summaries of the most recent research here--at least three required